EVALUATION OF NS1 ANTIGEN-CAPTURE ELISA AND IgM ANTIBODY CAPTURE ELISA IN ACUTE CASES OF DENGUE IN RURAL POPULATION IN A TERTIARY CARE TEACHING HOSPITAL IN WESTERN UTTAR PRADESH INDIA
Background: Dengue is a major public health problem in resource-limited countries like India. There has always been a quest for best serological test for prompt and accurate diagnosis and whether single test or a combination be used. Enzyme immunoassays based on the detection of dengue virus non-structural protein 1 (NS1), and IgM antibody capture are available with different sensitivities and spe...
Exercising in a gym, largely aimed at getting a better-shaped body, is a long and tiring process. So many people opt for power enhancing drugs, boosting their athletic performance. These include steroids, erythropoietin, creatine, protein and zinc supplements among others. Together with increasing the muscle bulk, they exert highly undesirable effects on normal physiology. The subjects were asked ...
DEVELOPMENT OF RP-HPLC METHOD FOR THE ESTIMATION OF IN VITRO AND IN VIVO SAMPLES OF KETOPROFEN IN BULK DRUG AND TRANSDERMAL DOSAGE FORM
A simple and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of ketoprofen in vitro samples and in vivo samples from bulk drug and from administered transdermal dosage form, using Spherosorb S5 ODS of 10cm X 4.6 mm column, (5μm particle size). Mobile phase for in vitro sample analysis and in vivo plasma samples consists of 0.01 ...
Objective: The present work is aimed to screen phytochemicals, in vitro antimicrobial and antioxidant activity of the non polar and polar extracts of Acacia sinuata. Methods: Leaves of Acacia sinuata- were collected, dried and powdered. The powdered material is subjected to soxhlet extraction using various polar to non polar solvents and allowed for evaporation. The crude extracts thus obtained we...
HEXAVALENT CHROMIUM INDUCED ALTERATIONS IN THE NUCLEIC ACIDS AND PROTEIN METABOLISM IN THE LIVER OF THE FINGERLINGS OF FRESHWATER SILUROID, MYSTUS (M.) VITTATUS (BL.)
Fingerlings of Mystus (M.) vittatus (Bl.) a freshwater siluroid when subjected to a sublethal concentration of 2.4 mg/l hexavalent chromium for 20 days elicited significantly decreased RNA and protein in the liver. However, 10 days of exposure under the same stress produced no significantly alteration neither in the protein content nor in the nucleic acids (DNA, RNA) content of the liver. Results ...
The present study was undertaken to investigate the hepatotoxic potential of rosiglitazone- an oral hypoglycemic thiazolidenedione class of drug. The hepatotoxic potential of rosiglitazone was assessed in-vitro on isolated rat hepatocytes before subjecting to in-vivo studies. The in-vitro activity was measured by trypan blue exclusion assay and determination of liver marker enzymes. The hepatotoxi...
NOOTROPIC ACTIVITY OF ISORHAMNETIN IN AMYLOID BETA 25-35 INDUCED COGNITIVE DYSFUNCTION AND ITS RELATED mRNA EXPRESSIONS IN ALZHEIMER’S DISEASE
Oxidative stress appears to be an early event involved in the pathogenesis of Alzheimer’s disease. The present study was designed to investigate the neuroprotective effects of isorhamnetin (IRN) against amyloid beta 25-35 (Aβ 25-35)-induced memory impairment and oxidative damage in rats. Memory task was assessed using Y-arm maze and it revealed the impairment in spatial memory. The IRN treated ...
DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF GLIMEPIRIDE IN TABLET DOSAGE FORM
An accurate, sensitive and precise RP-HPLC method has been developed and validated for the estimation of Glimepiride (GLM) from bulk drug and Pharmaceutical Dosage form. The separation was achieved by Hypersil C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Buffer in proportion of 60:40v/v, buffer was 0.02M Potassium Di-hydrogen Phosphate (pH 4.5 a...
IMPACT OF AN EDUCATIONAL INTERVENTION ON ASTHMA KNOWLEDGE AND METERED-DOSE INHALER TECHNIQUE AMONG NURSING STUDENTS OF GOVERNMENT COLLEGE OF NURSING, BENGALURU
Nurses being one of the important members of the healthcare team, play a significant role in providing care and education. Current effective treatments for asthma are in the form of metered-dose inhalers. An effective control is achieved only when the patient is comfortable to use the metered-dose inhaler device correctly. This study was therefore planned to evaluate their knowledge and to assess ...
THE EFFECT OF UGT1A6 POLYMORPHISM AT TWO LOCI ON THE CLINICAL RESPONSE TO VALPROIC ACID IN EPILEPTIC CHILDREN
Background and Aim: Valproic acid (VPA) is a widely used antiepileptic drug. The main pathway of its elimination is through conjugation by UDP-glucuronosyltransferases with several polymorphic forms. In this study, we aimed to examine the effect of polymorphism in UGT1A6 enzyme-coding gene at two loci, namely, 541A>G and 552A>C on clinical outcome in Egyptian children with idiopathic epileps...