APPLICATION OF EXTRACT OF GERANIUM PALUSTRE HERB AS A DENTAL FILM IN THE TREATMENT OF ORAL MUCOSA DISEASESAbstract
Investigation of antioxidant, anti-inflammatory and antimicrobial activity of a dental film, which easily reabsorbs with applied phytocomplex of Geranium palustre, has been performed to use it for the treatment of oral mucosa diseases. To get the extract, the dried material was grounded, extracted and lyophilized. Quantitative determination of biologically active substances in lyophilized phytocomplex was performed on spectrophotometer and high performance liquid chromatograph. For investigation of antioxidant properties of the extract, the method, based on the interaction with stable chromogen-radical DPPH, was used. Investigation of anti-inflammatory activity of a dental film with extract of Geranium palustre herb was carried out based on carrageenin model of inflammatory. The study of antimicrobial properties of extract was performed using laboratory strains of microorganisms as a test-culture. The obtained qualitative indices of biologically active substances of the investigated phytocomplex showed high content of tannins, polyphenol compounds, polysaccharides, phenolcarboxylic acids, organic acids, ascorbic acid, free amino acids and other compounds, due to which lyophilized extract possesses a wide range of properties, important for treatment: antioxidant, anti-inflammatory, antimicrobial and antiviral. The obtained dental film firmly adheres to the mucosa surface, has pH of the oral cavity and quickly reabsorbs, providing regular release of plant extract and convenient application. Moderate antioxidant, anti-inflammatory and antimicrobial activities as well as marked fungistatic action of lyophilized extract were detected. Fast reabsorbing dental film with applied Geranium palustre phytocomplex, possessing necessary properties for the treatment of oral mucosa diseases, is a safe and perspective remedy for implementation into dental practice.
M. Khаvrona *, I. Benzel, R. Fedin and O. Pinyazhko
Department of Pharmacology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
26 February, 2018
05 May, 2018
12 June, 2018
01 November, 2018