DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF DIPHENHYDRAMINE HYDROCHLORIDE IN SOFT GELATIN CAPSULEAbstract
A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of Diphenhydramine Hydrochloride in soft gelatin capsule dosage form. For UV spectrophotometric method, maximum absorption was found at λmax 258nm.The percentage recovery of Diphenhydramine Hydrochloride ranged from (98.97 ± 0.2989) in capsule dosage form. The developed method was validated as per ICH guidelines with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 10-100μg/ml having line equation y = 0.016x – 0.018 with correlation coefficient of 0.9934. By treating the data statistically and by recovery study, results of study were validated.
Arun Kumar Mishra *, Arvind Kumar and Amrita Mishra
Central Facility of Instrumentation, College of Pharmacy, IFTM, Moradabad, (UP), India
23 April, 2010
05 June, 2010
03 August, 2010
15 August, 2010