DEVELOPMENT OF Se DOPED ZnO NANOPARTICLES: ANTIMICROBIAL ACTIVITY AND IN VIVO ACUTE NANOTOXICOLOGICAL IMPACT ON SWISS ALBINO MICEAbstract
Selenium nanoparticles are attracting more and more attention due to their excellent photoelectrical characteristics and high biological activity. Selenium is also found to be effective against cancer. ZnO nanoparticles are reported to inhibit the growth of a wide range of pathogenic bacteria under normal visible lighting conditions. In this research work, we report the preparation of a set of Selenium doped ZnO nanoparticles (Zn1-xSexO1-δ; where x = 0.05, 0.10, 0.15 and 0.20) by simple chemical synthesis method. The materials were characterized systematically by X-ray Diffraction method, FT-IR spectroscopy, particle size analysis, SEM, EDAX analysis and TEM. The physical characterization studies have revealed the presence of fine particles in the prepared crystalline materials. The prepared materials were also subjected to antimicrobial assay by agar well diffusion method against both Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative bacteria (Escherichia coli and Klebsiella pneumoniae). Among studied, the material with the composition the materials Zn0.80Se0.20O1-δ have exhibited excellent antibacterial activity against the bacteria such as, Bacillus subtilis and Staphylococcus aureus. Considering the superior antimicrobial activity of Zn0.80Se0.20O1-δ, the study was further extended to assess the potential toxic effect of these materials in Swiss Albino mice through in vivo studies (by oral administration). From the histopathological studies, it was found that the mice treated with 2000mg/kgbwt of Zn0.80Se0.20O1-δ has shown mild central vein congestion in the hepatocytes due to the appropriate acute toxicity of Se doped ZnO nanoparticles at this high dosage level (LD50 ~ 2000 mg/kgbwt).
P. S. Kumar and A. S. Nesaraj *
Department of Chemistry, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.
13 September, 2017
14 December, 2017
30 December, 2017
01 June, 2018