DEVELOPMENT OF SIMULTANEOUS SPECTROPHOTOMETRIC METHOD FOR QUANTIFICATION OF RESVERATROL AND FOLIC ACID IN AN OROGELAbstract
A simple and precise UV-Visible spectrophotometric method for the simultaneous estimation of Resveratrol and folic acid in fixed-dose combination (FDC) has been successfully developed and validated. A suitable FDC of Resveratrol and folic acid was formulated for the treatment of oral squamous cell carcinoma. The present method involves the application of simultaneous equations (Vierodt’s method) for quantification of both the drugs. Resveratrol and folic acid were found to have absorbance maxima at 303.4 nm and 280.2 nm respectively in simulated saliva pH 6.8: methanol (1:1). The linearity studies indicated a concentration range of 1-7 ppm and 7-13 ppm for Resveratrol and folic acid respectively. The high values of the correlation coefficient indicate good linearity of the calibration curve for both the drugs. The recovery of Resveratrol and folic acid was found to be between 95.368 ± 0.364% and 101.260 ± 0.270% for Resveratrol and 101.330 ± 5.834% and 105.229 ± 0.1.164% for folic acid. The recovery results indicated that this method was suitable for simultaneous estimation of Resveratrol and folic acid in an FDC, i.e., mucoadhesive orogel.
T. Khan, S. Gharat, M. Chalke and M. Momin *
Department of Pharmaceutics, SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, Mithibai College Campus, Mumbai, Maharashtra, India.
16 July 2018
20 November 2018
28 March 2019
01 April 2019