EVALUATION OF ANTIANAPHYLACTIC ACTIVITY OF VARIOUS EXTRACTS OF WITHANIA SOMNIFERA IN RATSAbstract
Antianaphylactic activity of Withania somnifera was investigated by using rat peritoneal mast cells. The investigation was carried out by presensitization of the rat by using sheep serum and tripple antigen. Sensitization induces mast cell degranulation. The rats which were pretreated with standard drug, prednisolone (10 mg) and different extracts (petroleum ether, methanol and aqueous extract) of Withania somnifera at a dose of 250 mg, 500 mg and 750 mg were observed for the degranulation of mast cells, during the anaphylactic reactions. Treatment with methanolic extract of Withania somnifera at a dose of 500 mg shows beneficial effect on mast cell degranulation in sensitized rats. The activity was comparable with that of standard drug Prednisolone. Antianaphylactic activity of methanolic extract of Withania somnifera on the rat mesenteric mast cells may be possibly due to the membrane stabilizing potential.
C. Girish and Y. Narsimha Reddy *
Department of Pharmacology, University College of Pharmaceutical sciences, Kakatiya University, Warangal, Andhra Pradesh, India.
19 September, 2016
20 December, 2016
08 January, 2017
01 April, 2017