EVOLUTION OF AMINOGLYCOSIDE RESISTANCE IN CTX-M-15 PRODUCING KLEBSIELLA SPP. IN KURDISTAN PROVINCE, IRANAbstract
Background: the aim of present investigation was evolution of aminoglycoside resistance in CTX-M-15 producing Klebsiella spp. Clinical isolates from general hospitals of Kurdistan Province, Iran. Methods: Ninety Klebsiella spp. isolates were obtained from different clinical specimens. Antibiotic susceptibility pattern and detection of ESBL producing isolates performed by Kirby-Bauer disc diffusion method according to Clinical and Laboratory Standards Institute’s (CLSI) guidelines. CTX-M-15 gene screened by PCR amplification. Fisher tests was used to analyses by with STATA software program and p-values below 0.05 were considered as significant. Results: Out of 90 clinical isolates the highest and lowest resistance related to cefotaxime and imipenem respectively. The results of detection of ESBL producing isolates showed that 62 isolates (84.4%) were ESBL-positive isolates. Thirty two (42.1%) out of 90 Klebsiella spp. isolates caring CTX-M-15 gene. CTX-M-15 was detected in 56.2% of gentamycin resistance, 34.4% of kanamycin resistance, and 56.2% of amikacin resistance. Conclusion: the present study showed that there was high frequency of CTX-M-15 gene in ESBL producing clinical isolates in Kurdistan Province, Iran. Moreover, there was no statistically significant relationship between CTX-M-15 production and resistance to aminoglycoside antibiotics in Klebsiella spp. isolates.
P. Shakib, M. Taherikalani and R. Ramazanzadeh *
Cellular Molecular Research Center and Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran.
05 March, 2018
05 May, 2018
13 May, 2018
01 November, 2018