IN VIVO AND EX VIVO EVALUATION OF LUFFA ACUTANGULA FRUIT EXTRACT AND ITS FRACTIONS FOR HEPATOPROTECTIVE ACTIVITY IN WISTAR RATSAbstract
The alcoholic extract of fruits of the plant Luffa acutangula previously reported for is hepatoprotective activity was fractionated into three parts to chemically identify the most potent bioactive fraction. The hepatoprotective potential of the fraction prepared from extract was studied in vivo in rats as well as ex vivo in isolated hepatocytes against paracetamol induced hepatotoxicity. The hepatoprotective activity was determined on the basis of their effects on parameters like direct bilirubin, serum aspartate transaminase, serum alanine transaminase and alakaline phasphatase. Ethyl acetate fraction (EAF) of alcoholic extract 100 mg/kg, p.o.) exhibited maximum hepatoprotective activity and is the active fraction for hepatoprotective activity of L. acutangula fruit.
S. B. Mishra and A. Mukerjee*
Department of Pharmacognosy, United Institute of Pharmacy, Naini, Allahabad, Uttar Pradesh, India.
15 May, 2017
18 October, 2017
19 November, 2017
01 December, 2017