IN VIVO ANTI-INFLAMMATORY ACTIVITY OF ETHYL ACETATE EXTRACT DERIVED FROM MARINE STREPTOMYCES CARPATICUSAbstract
Anti-inflammatory activity of the SC-EA extract of the marine Streptomyces carpaticus was studied in Wistar rats using acute and chronic inflammatory models like carrageenan induced paw edema and cotton pellet induced granuloma models respectively. The acute toxicity of SC-EA extract was tested on adult Wistar rats. The maximum dose of SC-EA extract at 2000mg/kg does not show any sign of visual changes in physical behaviour, protein level and enzyme activities of AST, ALT, and ALP after 14 days of administration in rats. The SC-EA extract of 50mg/kg and100 mg/kg, p.o. demonstrated the inhibition of paw edema to 42.18% and 47.08% respectively and standard diclofenac showed 46.78% inhibition after 4 h of carrageenan treatment. In cotton pellet induced granuloma, maximum inhibition was accounted with SC-EA extract 58.3% at a dose of 100mg/kg and the suppression of inflammation was comparable to Diclofenac (30mg/kg), which reduced the weight of cotton pellet granuloma by 56.5%. The hydroxyproline which is a major component of the protein collagen was also estimated in SC-EA extract treated rats. This was measured as 17.3µg/mg with SC-EA extract at 100mg, whereas Diclofenac group showed 15.60µg/mg tissue.
Y. Latha, Vasavithirumalanadhuni, V. Mathakala and P. U. M. Devi*
Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam (Womens University), Tirupati, Andhra Pradesh, India.
19 April, 2017
17 June, 2017
29 June, 2017
01 December, 2017