SIMULTANEOUS DETERMINATION OF ETODOLAC AND PARACETAMOL IN BULK DRUGS AND PHARMACEUTICAL FORMULATION BY HPTLC-DENSITOMETRIC AND UV-DERIVATIVE SPECTROPHOTOMETRY METHODSAbstract
In current research work two methods have been developed for simultaneous determination of etodolac and paracetamol in binary mixtures. HPTLC-densitometry was employed for the determination of the mixture for etodolac 50-400 ng band⁻¹ and for paracetamol 50-300 ng band⁻¹. Separation was carried out on a silica gel 60 F254 HPTLC plates, using toluene: acetone: methanol: glacial acetic acid (6:2:1:0.5 v/v) as mobile phase. Etodolac, paracetamol and the toxic impurity para-aminophenol were well resolved with Rf values of 0.61±0.04, 0.41±0.04 and 0.20±0.03. Determination has been carried out at 254 nm with a mean percentage recovery of 99.77±1.30 for etodolac and 99.86 ± 0.97 for paracetamol. First derivative (D1) spectrophotometry was employed for simultaneous determination of etodolac (217nm) and paracetamol (236 nm). Linearity ranges of both the compounds were found to be 2.5-12.5µg mL⁻¹ with a mean percentage recovery of 98.07±1.62 for etodolac and100.65±0.84 for paracetamol respectively. Methods were validated according to ICH guidelines and successfully implemented for the analysis of bulk powder and pharmaceutical formulations.
Kalakotla Shanker *, Sanjay Kumar Kuna and Shashidhar Purra
Centre for Pharmaceutical Sciences, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, Telangana, India.
03 October, 2016
09 December, 2016
16 December, 2016
01 May, 2017