A SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF FEBUXOSTAT IN BULK AND PHARMACEUTICAL FORMULATIONSAbstract
A simple and cost effective spectrophotometric method is described for the determination of febuxostat in pure form and in pharmaceutical formulations. The drug was highly soluble in methanol so it was selected as the solvent system for the drug. This ensured adequate drug solubility and maximum assay sensitivity. The linearity range for febuxostat at its wavelength of detection of 315 nm was obtained as 0.2–15 µg/ml.The linear regression equation obtained by least square regression method, were Y =0.0802.X + 0.0036, where Y is the absorbance and X is the concentration (in µg/ml) of pure drug solution. The absorbance was found to increase linearly with increasing concentration of febuxostat, which is corroborated by the calculated correlation coefficient value of 0.9999. The limit of detection and limit of quantification was found to be 0.1743 µg/ ml & 0.5281 µg /ml respectively. The validity of the described procedure was assessed. Statistical analysis of the result shows high accuracy and good precision. The proposed method was successfully applied to the determination of febuxostat in pharmaceutical formulations without any interference from common excipients.
Paramdeep Bagga, Mohd Salman, H. H. Siddiqui*, Abdul M. Ansari, Tariq Mehmood and Kuldeep Singh
Faculty of Pharmacy, Integral University, Kursi Road, Lucknow, Uttar Pradesh, India
27 April, 2011
26 July, 2011
28 September, 2011
01 October, 2011