A VALIDATED ANALYTICAL METHOD FOR QUANTIFICATION OF CRISABOROLE IN MATRICES BY UPLC-ESI-MS/MSAbstract
The validated analytical method was applied for the estimation of Crisaborole in aqueous and human plasma with Crisaborole-D4 as an internal standard by using UPLC-ESI-MS/MS. The chromatographic separation was achieved with 10mM ammonium acetate buffer solution (pH- 4.5): Methanol, (10:90) (% v/v) using the Xterra C18, 100 × 4.6, 5µ. The total analysis time was 2 min, and the flow rate was set to 0.5 ml/min. The mass transitions of Crisaborole and Crisaborole-D4 obtained were m/z 252.1 ® 222.1 and 256.1 ® 222.1. The standard curve shows a correlation coefficient (r2) greater than 0.999 with a range of 75.00-225.00 ng/ml using the linear regression model.
T. M. Rao *, C. B. Reddy and P. S. Babu
Jawaharlal Nehru Technological University, Kakinada, Kakinada, Andhra Pradesh, India.
07 April 2019
04 July 2019
08 July 2019
01 August 2019