ACUTE TOXICITY TESTING OF SYNTHESIZED PYRAZOLINE DERIVATIVES IN ADULT ZEBRAFISHAbstract
Pyrazoline are reduction product of pyrazole. They are well known nitrogen containg 5-memberd heterocyclic compound with broad spectrum of biological activities such as antimicrobial, anti-inflammatory, anticancer, analgesic, anticonvulsant, anthelmintic, antioxidant and herbicidal. Pyrazoline derivatives have been synthesized from intermediate chalcone. Pyrazoles have been the recent target of numerous methodologies, mostly due to their prevalence as scaffolds in synthesis of bioactive compounds and reactions in different media. Fish are the widely used vertebrate organism in risk assessment and regulation. Out of them adult zebrafish and zebrafish embryo has a special characteristic which permits its use as a model organism in toxicological and pharmacological studies. Zebra fish known as a Danio rerio have risen as effective and powerful models for studying developmental processes and human disorders. Recently the zebrafish based assays have been developed for testing toxicity of drug candidates, including acute toxicity (LC50), organ specific toxicity and developmental toxicity. In present article the toxicity of newly synthesized pyrazoline derivatives has been studied on adult zebrafish and zebrafish embryos according to OECD guidelines for respective models.
P. M. Khedkar, D. M. Jagdale, S. R. Dhande*
University of Mumbai, Bharati Vidyapeeth’s College of Pharmacy, Mumbai, Maharashtra, India.
05 May, 2017
29 July, 2017
21 October, 2017
01 January, 2018