AN IN-VITRO PHAGE THERAPY – IN OPPOSITION TO FOOD POISON ENGENDERED STAPHYLOCOCCUS AUREUS VIA PRECISE BACTERIOPHAGE FROM TIRUNELVELI DISTRICT, TAMIL NADUAbstract
Staphylococcus aureus, the facultative anaerobic and opportunistic Bacterium has been characterized as the pathogen with diversified molecular adaptability leading to the emergence of MDR. Advanced use of alternate antibiotics is increasing the scenario of the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) to Oxacillin Resistant Staphylococcus aureus (ORSA) after that generating associated health infections. Even though Staphylococcus aureus is being considered as one of the important pathogens for its combination of virulence including toxin-mediated multidrug resistance and invasiveness, staphylococcus food poisoning is of high on interest as this infection rate is increasing day by day. Bacteriophage therapy has been of interest due to the absence of secondary resistance and targeted attack and also the lack of recolonization of the pathogen. The present study aims to isolate and purify Staphylococcus aureus-specific bacteriophages from sewage samples and thereby expunging food poison, causing Staphylococcus aureus. On this aspect Staphylococcus aureus causing food poison were isolated from a stool sample of patients at hospitals, and the specific Bacteriophage was isolated from the sewage in Tirunelveli District, India. Enrichment technique improved the stability of the phages. Spot and plaque assay experiments were done to examine the specificity of the isolated phage. Techniques employed result in providing purified specific bacteriophage for Staphylococcus aureus.
V. P. Kumar, D. S. Raj, M. Natarajan and S. Umamaheswari *
Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.
05 November 2018
18 February 2019
23 February 2019
01 July 2019