ANTI-BIOFILM ACTIVITY OF MIXED ‘TRANSITION METAL (Mn, Fe, Co, Ni, Cu AND Zn) – CALCIUM TARTARATE’ COMPLEXESAbstract
A series of six new, mixed transition metal and alkaline earth metal complexes of the general formulation [MM/(C4H4O6)2.xH2O] (where M= Mn, Fe, Co, Ni, Cu and Zn, M/ = Ca ) are synthesized by using bidentate tartarate ligand and are characterized by different analytical techniques such as elemental analysis, TGA, FTIR, XRD, SEM, magnetic susceptibility study, UV-visible spectroscopy etc. All synthesized mixed metal complexes (sample A1 to A6) were then tested for in vitro anti-biofilm activity against some fresh bacterial cultures namely of Pseudomonas aeruginosa ATCC-27853, E.coli ATCC-25922, Staphylococcus aureus ATCC-25923, Klebsiella pneumoniae (Lab culture), Proteus vulgaris (Lab culture). The Minimum Bactericidal Concentration (MBC) of these complexes was found slightly more than Minimum Inhibitory Concentration (MIC). It is found that the Biofilm Inhibition Concentration (BIC) levels of all the complexes are at higher side as compared to MIC but lower to MBC. The bio assays of all the complexes show a greater biofilm inhibition effect, than the individual tartarate ligand which indicates after the coordination the anti-biofilm activity of complexes is enhanced.
M. A. Betallu, V. B. Tadke, S. M. Vhankate, G. R. Pathade, A. G. Pathade and M. B. Ubale*
Department of Chemistry, Vasantrao Naik Mahavidyalaya Aurangabad, Maharashtra, India.
22 March, 2017
01 June, 2017
25 June, 2017
01 November, 2017