ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF ORAL DECOCTION OF PTEROCARPUS SANTALINUS BARK WOOD POWDER IN ACUTE INFLAMMATION MODELAbstract
Background: Raktachandan / Pterocarpus santalinus is mentioned to have edema reducing property in Ayurvedic literature. Studies have been performed using its methanolic extract for anti-inflammatory, analgesic and antioxidant activities. Since, Ayurveda advises to use it as a decoction, we planned to study the acute anti-inflammatory activity of decoction prepared from Pterocarpus santalinus bark-wood powder. Methodology: Albino rats of either sex were divided into 4 groups of 6 rats each (Group I – Control (CMC-vehicle), Group II – Ibuprofen suspension, Group III – P. santalinus suspension 3.5 mg/kg, Group IV – P. santalinus suspension 7 mg/kg. All the experimental animals were given standard and test drugs orally, 45 min before inducing inflammation. Acute inflammation was induced by injecting 0.1ml of 1% carrageenan solution in sub-plantar tissue of left hind paw of the rats. Paw volume (Plethysmometer) and pain assessment (Randall and Selitto paw withdrawal method) were done at 0 h (before medication), then at 1 h, 2 h, 3 h and 4 h after induction of inflammation. Data was analysed using Graph Pad Prism version 5. ANOVA followed by Tukey’s test was used for comparison among groups. Results: P. santalinus suspension 7 mg/kg and ibuprofen treated rats showed significant reduction (p<0.05) in their paw volume compared to the other groups. There was significant reduction in pain threshold (gm/sec) in all the groups (p<0.05) but in P. santalinus 7 mg/kg and Ibuprofen treated groups the pain threshold gradually increased after 2 h of induction of inflammation. Conclusion: Orally given decoction of Pterocarpus santalinus bark-wood powder in 7 mg/kg dose showed significant anti-inflammatory and analgesic activity in carrageenan induced inflammatory model in rats.
V. Ratnamraju, P. P. Dhande *, A. O. Gupta and N. S. Vaz
Department of Pharmacology, Bharati Vidyapeeth (Deemed to be University) Medical College, Dhankawadi, Pune, Maharashtra, India.
04 February, 2018
13 April, 2018
13 May, 2018
01 October, 2018