ANTIBACTERIAL POTENTIAL, PHYTOCHEMICAL INVESTIGATION AND CHARACTERIZATION OF ANTIBACTERIAL PROTEIN OF DIFFERENT FRACTIONS OF ACALYPHA INDICAAbstract
Medicinal plants are extensively used to cure various infectious diseases in human. Our present study was undertaken to investigate antibacterial activity in leaf, stem and root extracts of Acalypha indica against the human pathogens such as Staphylococcus sp, E. coli, Salmonella sp, Klebsiella pneumonia and Pseudomonas. The shade dried leaves, stem and root powder was used to prepare extracts by using and acetone, methanol and ethanol by soxhlet method. The antibacterial activity was studied by using agar well diffusion method with different concentrations of extracts (25, 50, 75 & 100µl). The result showed that ethanol leaf extract is effective against tested pathogen. Highest inhibition of zone was recorded in E. coli ( 22 cm). Phytochemical analysis of Acalypha indica was done. In the present study, the antimicrobial factors of A. indica were found to have Flavonoids, Triterpenoids, Phenolic compounds and proteins in leaf and its molecular weight was found to be 19kDa. Recognition of the molecular factor responsible for bactericidal activity in herbs will pave the way to commercialize it for disease management.
D. Kanchana Devi* and A. Vimal Raj
Department of Microbiology, Hindusthan College of Arts and Science, Coimbatore-28, Tamil Nadu, India
25 September, 2012
09 November, 2012
29 December, 2012
01 January, 2013