ANTIMALARIAL ACTIVITY AND TOXICITY EVALUATION OF THE ALKALOID-RICH FRACTION OF MOMORDICA CHARANTIA FRUITSAbstract
Introduction: We investigated to an in-vitro and in-vivo evaluation of antimalarial and toxicity of the alkaloid-rich fraction of Momordica charantia fruits. Materials and Methods: In-vitro and in-vivo antimalarial activity tests of Plasmodium falciparum strain 3D7 culture were performed on Plasmodium berghei-infected mice using suppressive and prophylactic methods, and evaluation of fraction toxicity was conducted on zebrafish (Danio rerio). Results and Discussion: The study found that IC50 value of the in-vitro test was 0.17 ± 0.12 µg/mL and anti-malaria test using a suppressive and prophylactic method at a dosage of 100 mg/kg/day was found to inhibit parasites by 74 and 73%, respectively. The evaluation of toxicity by the use of zebrafish revealed abnormalities in the formation of the heart (51.28%) and yolk sac (51.28%). Conclusion: Alkaloid rich-fractions have powerful in-vitro and in-vivo antimalarial activity, but they have teratogenic effects that can inhibit the formation of the heart and yolk sac.
S. Abdillah *, Y. Farida, Kartiningsih, N. M. D. Sandhiutami and K. Mohamad
Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, South Jakarta, Indonesia.
23 June 2018
01 April 2019
24 April 2019
01 May 2019