ANTIMICROBIAL SUSCEPTIBILITY TEST ANALYSIS OF AEGLE MARMELOS BARK EXTRACTSAbstract
India is a land of medicinal flora, there are large numbers of plants with miraculous medicinal values has been used traditionally to cure several diseases from decades in Ayurveda. So the present study was designated to evaluate the antimicrobial activities of Aegle marmelos (bale) bark extracts. In this study different bark extracts were prepared using various solvents, further these extracts were analyzed against selected microbial strains like Escherichia coli (MTCC-443), Bacillus subtilis (MTCC-441), Pseudomonas aeruginosa (MTCC-4673), Staphylococcus aureus (MTCC-3160), Aspergillus brasiliensis (MTCC-1344) and Candida albicans (MTCC-227) by agar well diffusion method and determination MIC by broth dilution method. The extracts showed a broad spectrum of antimicrobial activities by inhibiting the growth of respective microorganism in agar well diffusion assay. Extracts of acetone and ethanol 70% showed highest activity against Escherichia coli whereas methanol and choloroform extracts showed significant activity against Bacillus subtilis. The present study supports the immense medicinal properties of Aegle marmelos bark. The study may be insight for future researches in area of new drug development of herbal origins.
R. Dahiya, R. C. Choudhary, R. S. Tomar and V. Shrivastava *
Amity Institute of Biotechnology, Amity University, Gwalior, Madhya Pradesh, India.
07 October, 2017
13 February, 2018
19 February, 2018
01 August, 2018