ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACTS OF LEAVES AND FLOWERS OF NERIUM INDICUMAbstract
The Methanolic extract of leaves (LE) and Methanolic extract of flowers (FE) of Nerium indicum (Arali) was analysed for Antioxidant activity (AOA) in terms of DPPH free radicals, Total Phenolic Content (TPC) was measured in terms of Gallic acid equivalent and Flavonoid content was analysed in terms of Quercetin equivalent. The antioxidant activities of (LE & FE) were found to be 72.8% and 67 %. TPC of study extracts (LE & FE) were found to be 227 mg/100g and 449 mg/100g. The Total Flavonoid content of (LE & FE) were found to be 125 mg/100g and 199 mg/100g respectively. Superoxide anion radical scavenging activity of flowers was 66 % compared to leaves 25 %. Similarly Lipid Peroxidation showed higher activity in flowers than leaves. Enzymatic antioxidant activity such as Superoxide Dismutase, Glutathione peroxidase and Catalase of Nerium indicum flowers were around 10% to 30% higher than that of leaves. The results clearly indicate that the methanol extracts of Nerium indicum flowers have more potent antioxidant activity than leaves.
A. Vinayagam and P. N. Sudha
Department of Chemistry, DKM College for Women, Thiruvallur University , Vellore, Tamil Nadu, India
14 March, 2011
03 April, 2011
18 May, 2011
01 June, 2011