ANTIOXIDANT AND CYTOTOXICITY POTENTIAL OF ALCOHOL AND PETROLEUM ETHER EXTRACT OF BORASSUS FLABELLIFER LINN.Abstract
This study was subjected to investigate antioxidant and cytotoxic properties of ethanol and petroleum ether extracts of Borassus flabellifer Linn, expressed as BFA and BFP respectively. DPPH free radical scavenging method, total flavonoid, total phenol, total antioxidants and reducing power of the two extracts were assessed for antioxidant properties and cytotoxicity potentials were assessed by the brine shrimp lethality test. In DPPH test the lowest IC50 value was 32.59 mg/ml for BFA and highest was 1388.46 mg/ml for BFP and compared with standard ascorbic acid. In cytotoxicity test LC50 value was found 32.36 μg/ml for BFA and 616.59 μg/mlfor BFP. So alcohol extract is more potent than petroleum ether extract. The total Flavonoid contents of the test samples were 3.57 ± 1.26 and 17.41±1.89 mg/g quercetin equivalents for BFA and BFP respectively. The total phenol contents of the test samples were 707.08 ± 8.84 and 28.75 ± 6.48 mg/g galic acid equivalents for BFA and BFP respectively and also in total antioxidant assay BFA contains 0.68 ± 0.17 and BFP contains 527.38 ± 3.93 mg/g ascorbic acid equivalents. Borassus flabellifer may posses’ powerful antioxidant as well as good cytotoxic potential.
Shah Marzia Mahjabin Lina , K. M. Muhsinin Mahbub*, Imran Ashab , Md. Al-Faruk , Samiul Haque Atanu , Md. Jahir Alam and Masum Sahriar
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh
03 January, 2013
21 February, 2013
26 April, 2013
01 May, 2013