ANTIOXIDANT, ANTIBACTERIAL EFFECTS OF SOLANINE ISOLATED FROM SOLANUM NIGRUM AND ITS CYTOTOXIC ACTIVITY ON THE HEP-2 AND AGS CELL LINESAbstract
Solanum nigrum is a well known traditional medicinal plant. This study was intended to isolated active compound from the Solanum nigrum leaf. Solanine were isolated and purified from leaves of Solanum nigrum by using acid extraction along with alkaline precipitation methods and results shows that leaves of Solanum nigrum found to good sources of Steroidal glycol-alkaloids together with their structure were evaluated using various spectroscopic data of UV, FTIR, HPLC, NMR as well as MS. Anti-oxidative effects of solanine were tested in the in vitro (DPPH, superoxide anion radical and the hydroxyl radical) furthermore antibacterial activity were analysed against pathogens E. coli, Staphylococcus aureus, Salmonella typhi – A and Pseudomonas aeruginosa and its cytotoxicity effects were tested using Hep-2 and AGS cell line.
A. Rajalakshmi and A. Jayachitra*
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India
19 December, 2016
27 April, 2017
21 June, 2017
01 July, 2017