ASSESSMENT OF IN-VITRO ANTICANCER AND ANTIBACTERIAL ACTIVITIES OF CARCHORUS HIRSUTUS PHYTOMEDIATED OPTIMIZED NANOSILVERAbstract
The elected work reports for the first time successfully synthesized nanosilver from the aqueous leaf extract of the Carchorus hirsutus plant. Because the phytochemicals within the leaf extract were creditworthy for the reduction of silver ions as the active nanosilver particles, in addition to this, they act as the capping agents who had been environed at the surface of the particles. UV-Vis spectroscopy oversaw the establishment of nanosilver as an appropriate peak detected at 412 nm of surface plasmon resonance has affirmed the colloidal nanosilver. The peaks of Fourier Transform Infrared Spectroscopy had proved to be phytochemicals responsible for the reduction of nanosilver. The crystalline structure and average diameter found to be 29 nm of the phytosynthesized nanosilver particles were found out using X-ray diffraction studies. The Scanning Electron Microscopy images were rendered the aggregated, semi-spherical to spherical silver nanocrystals. The elemental signals of energy dispersive X-ray spectrum substantiate the presence of silver detail as silver weight % is 86.84 and atomic % is 68.45. The atomic force microscope turned into used to take a look at the dimensions, topographical structure of the synthesized nanosilver. Then the antibacterial activity of synthesized nanosilver has been effectively examined by the well-diffusion method in opposition to four harmful bacteria Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas putida, and Bacillus subtilis as evincing high activity. The Corchorus hirsutus mediated nanosilver exerts their cytotoxic impact on MCF7 cell lines of breast adenocarcinoma cells and HeLa cell lines of cervical cancer cells.
D. Ashok *, S. Raju and K. Rama
Green and Medicinal Chemistry Laboratory, Department of Chemistry, Osmania University, Hyderabad, Telangana, India.
25 January 2019
24 April 2019
14 June 2019
01 October 2019