BIOSYNTHESIS OF COPPER OXIDE NANOPARTICLES USING CYANOBACTERIA SPIRULINA PLATENSIS AND ITS ANTIBACTERIAL ACTIVITYAbstract
Recently, biological synthesis of nanoparticles (NPs) has attracted attention because of the need to develop new cost-effective and efficient synthesis protocol. In this paper, we account for the use of cell-free extract of Spirulina platensis in the biosynthesis of copper oxide nanoparticles (CuO NPs) of dimensions 30 – 40 nm. The biosynthesised CuO NPs were confirmed visually by appearance of dark brown colour formation in mixture and surface plasmon resonance band observed at 259 nm by using UV-Visible Spectroscopy. The size and morphology of the nanoparticles were confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. Furthermore, these NPs were found to exhibit high antibacterial activity against pathogenic gram-negative i.e. Escherichia coli – MTCC-9721, Proteus vulgaris – MTCC-7299, Klebsiella pneumonia – MTCC-9751 and gram-positive i.e. Staphylococcus aureus – MTCC-9542, S. epidermidid -MTCC- 2639, Bacillus cereus – MTCC-9017 bacteria. The CuO NPs had shown maximum zone of inhibition (ZOI) i.e. 28.0 ± 0.41 mm in P. vulgaris.
S. Saran, G. Sharma, M. Kumar and M. I. Ali*
Department of Biosciences, Suresh Gyan Vihar University, Jaipur, Rajasthan, India.
13 February, 2017
10 April, 2017
26 April, 2017
01 September, 2017