CHARACTERISTIC AND OPTIMIZED USE OF BIOACTIVE COMPOUNDS FROM GLORIOSA SUPERBA AND ALBIZIA AMARA WITH APOPTOTIC EFFECT ON HEPATIC AND SQUAMOUS SKIN CARCINOMAAbstract
Background: Cancer is still a dreadful disease, the treatment ranges with difficulties like strong side effects, shortage of donor and organs. In relation, plants and its chemical properties will provide an efficient source in the innocuous treatment for cancers. Objectives: To identify and isolate bioactive compounds from Gloriosa superba tubers and Albizia amara leaves with anti-cancer property that has traditionally been suggested as an anti-proliferative agent in ethnomedicine. Design: G. superba tubers and A. amara leaves are extracted and screened qualitatively. Extracts were then tested for antioxidant by DPPH assay. Highly potential extract with antioxidant are preceded to GCMS. The compounds determined from GCMS were selected according to its non-reported bioactive information on PubChem, and preceded against Hep-G2 and A431 cancer cell by MTT assay. Result: Ethanolic extract showed high antioxidant activity in both the plant extracts, GC-MS analysis was performed with ethanolic extracts revealing ample of phytoconstituent. Six non-reported bioactive compounds were isolated and identified using GC-MS. MTT assay was performed using isolated compounds such as 3-Hydroxy-4-methoxymandelic acid, 1-Butanone,1-(2, 4, 5 trihydroxy phenyl), 2H-1-Benzopyran, 3, 5, 6, 8 tetrahydro, β-Amyrin trimethylsilyl ether, Undecane, 2, 8-dimethyl and Octadecanoic, 2-oxo methyl ester on Hep-G2 and A431 cell lines. The optimized use of these compounds reveals apoptotic growth inhibition of 50.58% in A431 cell line and 53.42% in Hep-G2 cell line. Conclusion: The anti-cancer property of G. superba tubers and A. amara leaves as mentioned in various ethnopharmacological records, concurrently emphasize the possible bioactive compounds to treat the hepatic and squamous skin carcinoma.
F. A. Jayakumar*, S. E. Simon, Y. W. Yan and P. W. Yap
Department of Biosciences, School of Science and Engineering, Malaysia, University of Science and Technology (MUST), Kota Damansara, Petaling Jaya, Selangor, Malaysia.
28 July, 2017
13 September, 2017
17 September, 2017
01 May, 2018