CHARACTERIZATION AND CYTOTOXICITY EVALUATION OF SUPERPARAMAGNETIC NANOPARTICLES BIOSYNTHESIZED BY FUSARIUM OXYSPORUM SKAbstract
Superparamagnetic iron oxide nanoparticles have been used for various industrial and clinical applications. In the present study, biosynthesis of superparamagnetic Fe3O4 nanoparticles was carried out using a fungal isolate. The fungal isolate was identified as Fusarium oxysporum SK through 18S ribosomal DNA sequence analysis. The synthesized magnetic nanoparticles were subjected to Transmission Electron Microscopy (TEM) and Scanning Electron microscopy (SEM) to measure the morphology and size of the nano particles. The topology was assessed by X-ray Diffraction (XRD) analysis and the magnetic property was assessed by Vibrating sample magnetometry (VSM). The nanoparticles were spherical in shape and the average size was measured as 15nm. The VSM data showed that the nanoparticles were superparamagnetic and the saturation magnetization was found to be 19.9emu/g. Since the particles were synthesized for the application in biomedical field, the particles were tested for toxicity against human monocytes through MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay. The MTT assay results confirmed the particles are nontoxic.
I. Arulpandi and S. Kanimozhi *
P. G. & Research Department of Microbiology, Asan Memorial College, Chennai – 600100, Tamilnadu, India.
31 May, 2014
25 July, 2014
02 October, 2014
01 January, 2015