CYTOTOXIC ACTIVITY OF CROCUS PALLASII SUBSP. HAUSSKNECHTII IN HUMAN CANCER CELL LINESAbstract
Crocus pallasii subsp. haussknechtii grows in the west of Iran where local people consume the corm. It belongs to a saffron family (Iridaceae), which has shown several biological activities including cytotoxic and anti-angiogenesis properties. The corm (the nut and the surrounding fibers) and aerial parts of the species have shown anti-angiogenesis properties in HUV-EC-C cells previously; in the present study, the cytotoxic activity of different parts of the plant was evaluated. As people consumed the nuts, the aerial parts, nuts, and fibers were dried and ground and extracted separately. The methanol extract and petroleum ether, chloroform and methanol fractions were prepared by maceration and evaluated in A-549, HepG-2, MCF-7 and HT-29 cancer cell lines through MTT assay. The aerial parts showed no toxicity to any of the cancer cell lines up to the concentration of 100 μg/mL. The methanol extract and fraction of the fibers showed cytotoxicity to all cell lines while the methanol extract and fraction of the nuts and the chloroform fraction showed cytotoxicity to HepG-2 and A-549 cells, respectively.
M. Hamzeloo-Moghadam *, R. Shahrestani, B. Keramatian and S. Mohebby
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
29 April 2018
23 June 2018
26 June 2018
01 January 2019