CYTOTOXIC AND ANTIVIRAL ACTIVITY OF RIBES UVA CRISPA LINN. AND RIBES MULTIFLORUM KIT. EX ROMER AND SCHULTES EXTRACTSAbstract
ABSTRACT: To find new antiviral agents from natural sources, methanol and aqueous extracts of leaves and fruits of Ribes uva –crispa and Ribes multiflorum naturally grown in Turkey were investigated as in vitro to reveal their antiviral activities against Herpes simplex virus type 1. All experiments were performed in 96-well plates and results were measured with XTT-based colorimetric assay. Results demonstrated that all extracts have no cytotoxic effect on vero cells at 10000 µg/mL, while they also exhibited anti-HSV-1 activity with different percentages of protection (varying between 2.65% – 50.40%) in 10000 µg/mL which was at the highest concentration in vero cells. 50% effective concentrations (EC50) of the extracts which were determined having percentage of protection against HSV-1 at concentrations lower than 10000 µg/mL were calculated using GraphPad Prism Version 5.03 statistics program with non-linear regression analysis. These extracts were determined to have EC50 values ranging between 9710 – 70600 µg/mL and selectivity index (SI) are ranging between 0.14 – 1.03. On the basis of these results we believe that it would be worthwhile expanding these studies to include additional species of Turkish plants
R. Duman, H. H. Doğan *, M. Dinç and P. Tuncer
Department of Biology, Faculty of Science, Selçuk University, Konya, Turkey.
07 August, 2017
13 October, 2017
20 October, 2017
01 May, 2018