CYTOTOXICITY AND IN-SILICO STUDIES OF ANETHOLE IN TRIPLE NEGATIVE BREAST CANCER CELLSAbstract
Triple negative breast cancer (TNBC) is a type of breast cancer in which receptors such as estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 are absent. Triple-negative breast cancer is a high risk breast cancer that lacks the benefit of specific therapy that targets these receptors. In the present study, anethole an aromatic compound from star anise was exposed to TNBC cell line MDA-MB-231 to assess its anticancer property. Cytotoxicity assay was done to determine and optimize the dose. DNA damage was analyzed by DNA fragmentation method. The use of targeted agents against TNBC such as poly-ADP-ribose and insulin like growth factor receptor were investigated in the present study. The drug-target interaction was investigated using in-silico docking studies. The in-silico study provides evidence for the interaction of anethole with the target proteins namely poly ADP-ribose polymerase (PARP) and insulin like growth factor receptor (IGFIR). This interaction is presumably vital in exerting the anticancerous activity. The present study clearly elucidates that anethole possesses anticancer activity against the TNBC.
S. Sumathi *, A. Poornima, D. Muthukumari and P. R. Padma
Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, India.
23 June, 2017
05 June, 2018
08 July, 2018
01 August, 2018