EFFECT OF ETHANOLIC EXTRACT PROPOLIS TRIGONA SPP. MALANG INDONESIA ON ISOLATE STAPHYLOCOCCUS AUREUS BIOFILM ARCHITECTURE FROM CHRONIC RHINOSINUSITIS A CONFOCAL LASER SCANNING MICROSCOPIC STUDYAbstract
Biofilm in chronic rhinosinusitis (CRS) is the infection by bacteria, which is difficult to overcome. It has recurrent infections, mucosal inflammation, and postoperative symptoms. Propolis is a natural product that is potential as an anti-biofilm choice. The purpose of this study was to determine the ethanolic extract propolis (EEP) effect on the morphology of Staphylococcus aureus biofilm. The purpose of this study was to determine the effect of EEP on the morphology of Staphylococcus aureus biofilm. Isolate Staphylococcus aureus was taken from meatus medius of CRS patients in endoscopy sinus surgery at PHC Hospital Surabaya, Indonesia. Identification of Staphylococcus aureus uses Mannitol Salt Agar, Gram Staining, Catalase test, and Coagulase test. Biofilm produced from congo red agar culture. EEP was macerated from alcohol 70%, and after that the biofilm formed put in 24 well culture plate for 48 h using EEP solution dosages of 0.0%, 0.2%, 0.4%, 0.8%, 2.0%, 8.0%, 10.0% and negative control. Measurement intensity of expression Syto9 and the depth of biofilm using Confocal Laser Scanning Microscopy (CLSM) magnification is 400x. There were observed for 3 times field of view well. Morphology of Staphylococcus aureus biofilm was assessed by a decrease in the intensity of expression Syto9 and depth of biofilm. Based on the Kruskal Wallis test, the results showed that there were significant differences in the intensity of Syto9 expression p 0.001 <α 0.05 and depth of biofilm p 0.001 <α 0.05. In the Post Hoc test, EEP Trigona sp. 2.0% -10.0% inhibits biofilm growth.
W. Hadi, E. Handoko, Noorhamdani and S. R. Prawiro *
Department of Clinical Microbiology Faculty of Medicine, University of Brawijaya, Malang.
26 September 2018
02 January 2019
13 February 2019
01 June 2019