ESTIMATION OF (-)-HYDROXYCITRIC ACID (HCA) IN GARCINIA LANCEAEFOLIA ROXB. USING NOVEL HPLC METHODOLOGYAbstract
ABSTRACT: (-)-Hydroxycitric acid (HCA) has been found to be the principal acid in genus Garcinia, which is a potential metabolic regulator of anti-obesity activity and inhibitor of lipogenesis. Presence of (-)-HCA as the principal acid has added importance to thegenus Garcinia. The present study deals with extraction and estimation of (-)-HCA from Garcinia lanceaefolia,collected from four administrative districts of Assam, NE India. An improved HPLC methodology has been developed in the present study for estimation of (-)-HCA. Detection of (-)-HCA was done by observing the orange spot on paper chromatography. The Rf values were observed at 0.34 and 0.45 for free HCA and HCA lactone, respectively. Estimation of (-)-HCA was done by HPLC analysis, using water as mobile phase by taking UV detection at 210 nm and has been found to be 4.56 ± 0.13gm/10gm of dried sample. This is the first report of its kind on Garcinia lanceaefolia Roxb.
Ankur Gogoi *, Nabajyoti Gogoi and Bijoy Neog
Ph.D. Scholar, Centre for Studies in Biotechnology, Dibrugarh University, Dibrugarh- 786004, Assam, India
23 April, 2014
12 June, 2014
31 July, 2014
01 November, 2014