ESTIMATION OF OFLOXACIN IN BULK AND TABLET DOSAGE FORM BY NANODROP SPECTROPHOTOMETRIC METHODAbstract
Ofloxacin is a fluoroquinolone antibiotic considered to be a second-generation fluoroquinolone. Ofloxacin is administered by oral, intravenous or topical (eye and ear drop) route. It is used as antibacterial for the treatment of various diseases like chronic bronchitis, pneumonia, urethritis, cervicitis, urinary tract infections, prostatitis, uncomplicated urethral and cervical gonorrhea. Ofloxacin is a pale yellow or bright yellow, crystalline powder. It is slightly soluble in water, soluble in glacial acetic acid, slightly soluble or soluble in methylene chloride, slightly soluble in methanol. A simple Nanodrop spectrophotometric method was developed for the determination of Ofloxacin in pure and its pharmaceutical tablet dosage form. Ofloxacin exhibiting λ max at 296 nm in mobile phase (0.05 M Phosphate buffer: Acetonitrile) in ratio of 65:35 and obeyed linearity in the concentration range of 1- 150 ppm. The proposed method was statistically validated.
Rakesh K. Singh*, Pankaj S. Patel, Darshika Awasthi ,Bipin Bihari and Prabodh Shukla
College of Pharmaceutical Sciences *, Mohuda, Berhampur (Orissa), India
29 January, 2010
15 March, 2010
25 March, 2010
01 April, 2010