ESTIMATION OF QUERCETIN CONTENT IN THREE DIFFERENT SPECIES OF EUPATORIUM BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHYAbstract
Three different species of Eupatorium namely E. glandulosum, E. odoratum and E. triplinerve belongs to the family Asteraceae are selected for the analysis of quercetin content. Leaves are extracted with ethanol and water and used for the analysis of quercetin by HPTLC technique using the mobile phase containing toluene: ethyl acetate: formic acid: methanol (5.5:4:1:0.5). Determination of quercetin content was performed by densitometric scanning under 254 nm, and the quercetin was detected at the Rf value of 0.54. The quantity of the quercetin content in plant extract was estimated by the calibration curve obtained from the standard quercetin. The result showed that the ethanolic extracts of E. glandulosum showed a high amount of quercetin (17.44 mg/g) followed by E. odoratum (13.4 mg/g) and E. triplinrve (9.29 mg/g).