EVALUATION OF ANTIDIABETIC POTENTIAL OF WITHANIA SOMNIFERA BY GLUCOSE UPTAKE ASSAY ON 3T3 F442A FIBROBLAST (ADIPOCYTE) CELLSAbstract
Antidiabetic potential of leaf and root extracts of Withania somnifera from Nimuch, Lucknow, Mumbai and Karnataka regions of India were evaluated against 3T3F442A fibroblast (3T3 adipocyte) cell line using glucose uptake assay. Leaf extracts from all the four regions were active on the cell line tested. Samples from Mumbai region found to be more active when compared with other regions. Root extracts also gave good glucose promoting activity in the presence of insulin. Nimuch samples were mere active than Mumbai samples. Isolated fractions and standard Withaferin-A and Withanolide-A were active at 20 µg/ml. This concentration was higher than that for crude leaf extract from Mumbai region and crude extracts from Nimuch region. Crude extract of Withania somnifera have good antidiabetic potential indicating the synergistic effect of these extract on glucose uptake of 3T3 fibroblast in presence of insulin.
R. A. Shah *, S. Khan, M. Vakil and M. T. Qureshi
Department of Botany, The Institute of Science, Mumbai, Maharashtra, India
01 July, 2016
03 November, 2016
26 November, 2016
01 January, 2017