EVALUATION OF ANTIMICROBIAL ACTIVITY OF MYCELIA AND SPOROCARP OF ENTOLOMA SPECULUMAbstract
Antimicrobial activity of mycelia and Sporocarps has been adequately worked out but comparative studies of both has not done in detail. The present work is to compare the antimicrobial activity of mycelia and sporocarps of Entoloma speculum. It is a saprophytic, white fleshy fungi, belongs to Entolomataceae. The collected fruiting bodies were dried, powdered and subjected for secondary metabolites extraction. Mycelia was cultured by loading the spore loading the spore to potato dextrose agar plates. Mass culture of mycelia was done on patato dextrose broth (Shittu 2005) and was extracted by methanol. Both extracts were tested against pathogens, viz., Xanthomonas campestris, Pseudomonas syringae, Agrobacterium tumefaciens, Klebsiella pneumonia, Escherihia coli, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Trichophyton rubrum, Chrysosporium keratinophilum, Fusarium solani, Penicillium chrysogenum, Aspergillus niger. Mycelial extract inhibited T. rubrum maximum (36 mm), Sporocarp inhibited F. solani at 28mm maximum, the mycelial extract inhibited pathogenic fungi and sporocarps extract inhibited bacterial pathogens.
J. K. Kodiyalmath* and M. Krishnappa
Department of P.G. Studies and Research in Applied Botany Kuvempu University, Shankaraghatta, Shimoga, Karnataka, India.
07 June, 2017
26 August, 2017
29 August, 2017
01 March, 2018