FIRST DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF FLUOXETINE HYDROCHLORIDE AND OLANZAPINE IN TABLETSAbstract
Fluoxetine hydrochloride (FH) in combination with Olanzapine (OZ) is used in treatment of depressive episodes associated with bipolar disorder. The first derivative spectrophotometric method for simultaneous determination of fluoxetine hydrochloride (FH) and olanzapine (OZ) from two component tablet dosage form has been developed and validated. Chromatographic methods viz. HPLC and HPTLC methods were reported in the literature for the simultaneous determination of FH and OZ from combined dosage forms. In the present investigation an attempt has been made to develop accurate, reproducible, rapid and cost-effective method for simultaneous determination of FH and OZ in pharmaceutical formulation. The wavelengths selected for determination of FH and OZ is 235.5nm and 296nm, respectively. The beer’s law was obeyed in the concentration range of 8-80μg/mL and 2-20μg/mL for Fluoxetine and Olanzapine, respectively. The proposed methods were validated and successfully applied to the determination of FH and OZ in tablet formulations. The results suggested that the proposed procedures can be used for routine quality control of tablets containing FH and OZ.
Vijaykumar K. Parmar*, Jatin N. Patel , Girish K. Jani , Laxman M. Prajapati and Jagbir Gagoria
Assistant Professor, Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar- 388 120, India
29 July, 2011
15 September, 2011
25 October, 2011
01 November, 2011