GC-MS ANALYSIS OF CALLUS AND LEAF EXTRACTS OF IN VITRO PROPAGATED PLANTS OF JUSTICIA WYNAADENSIS (NEES) T. ANDERSONAbstract
The present study was aimed at induction of callus, micropropagation of Justicia wynaadensis (Nees) T. Anderson in vitro by using different explants and study of the phytocomponents present in the aqueous and methanol extracts of callus and leaf of in vitro propagated plants through GC-MS analysis. Callus was induced using MS basal medium supplemented with combinations of 2, 4-D, IAA and NAA growth hormones in various concentrations. For micropropagation hormone free MS basal medium and MS basal medium with BAP in various concentrations was used. Callus were obtained on MS medium supplemented with 2mg/L 2, 4-D. In vitro shoots were initiated on hormone free MS basal medium and well developed multiple shoots were regenerated on MS basal medium containing 1mg/L BAP. Four different extracts were prepared from callus and leaf of in vitro obtained plants and their phytochemical composition were analyzed by GC-MS. The extracts revealed the presence of various phytocomponents, with therapeutically important properties.
C. D. Vandana*, K. N. Shanti and S. L. Shantha
Department of Biotechnology, PES Institute of Technology, Bangalore, Karnataka, India.
17 May, 2017
17 July, 2017
25 July, 2017
01 February, 2018