HEPATOPROTECTIVE ACTIVITY OF METHANOL EXTRACT OF FENUGREEK SEEDS ON RATSAbstract
The present study was conducted to evaluate the hepato-protective activity of methanol extract of seeds of Trigonella foenum graecum (TFG)in CCl4 induced toxicity in Wistar albino rats. Seeds of TFG were collected, and subjected to continuous cold maceration in a macerator, for 72 h with solvents like petroleum ether (60 – 80) and methanol separately. Hepatic injury in rats was induced separately by administration of equal mixture of CCl4 and olive oil (50% v/v, 1.25 ml/kg, i.p.). Liver damaged was monitored by raised biochemical marker enzymes (SGOT, SGPT, and alkaline phosphatase). CCl4 was administered twice a week, on every first and fourth day of all 14 days. The extract at the dose of 250 mg/kg b. wt. was evaluated by inducing hepatotoxicity with CCl4 and using silymarin (100 mg/kg) as the reference standard. Biochemical parameters like, SGOT, SGPT and serum bilirubin level were analysed. A section of liver was subjected to histopathological studies. Based on the above studies, it is reported that the methanol extract of TFG possess significant hepato-protection against CCl4 induced hepatotoxicity in albino rats.
Department of Pharmaceutical Chemistry, NSHM College of Pharmaceutical Technology, NSHM Knowledge Campus Kolkata group of Institution, Kolkata-700053, West Bengal, India
17 November, 2013
19 December, 2013
26 March, 2014
01 April, 2014