HOMOLOGY MODELING AND ANALYSIS OF STRUCTURE PREDICTIONS OF KLF8 PROTEIN FROM HOMO SAPIENSAbstract
KLF proteins are highly conserved among mammals from human to mouse, with many KLFs also having homologs in Gallus gallus (Chicken), Danio rerio (Zebra fish), and Xenopus laevis (Frog). On the basis of functional characteristics, KLF proteins can be divided into three distinct groups. KLFs in group 1 (KLFs 3, 8, and 12) serve as transcriptional repressors through their interaction with the carboxy-terminal binding protein (CtBP). Family members in group 2 (KLFs 1, 2, 4, 5, 6, and 7) function predominantly as transcriptional activators. KLFs in group 3 (KLFs 9, 10, 11, 13, 14, and 16) have repressor activity through their interaction with the common transcriptional corepressor Sin3A. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A homology modelling method was used for the prediction of the structure and other various physico-chemical properties of protein were obtained using ProtParam. 3D structure was constructed for the target protein usingI- TASSER and SWISS-MODEL. The comparison between the structures generated from above mentioned tools indicates greater acceptability of the structure generated from I- TASSER and validation of structure was performed by using Ramachandran plot. The present study gave an outlook on KLF8 protein and further research to be carried out in preventing the cancer in human.
Vinod Kumar Jatav*, Lovekesh Parasher, Ankit Raje and Samraddhi Tiwari
Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, Madhya Pradesh, India
24 October, 2013
22 November, 2013
13 February, 2014