HPLC STANDARDIZATION OF ETHYL ACETATE EXTRACT OF FAGOPYRUM ESCULENTUM MOENCH. SEEDS AND IN-VITRO ENZYME INHIBITORY ACTIVITIESAbstract
Fagopyrum esculentum Moench. is a versatile herb as beyond its nutritional properties it also have various health benefits like anti-diabetic, anti-inflammation, anticancer, antibacterial, anti hypertensive etc. Most of these activities can be attributed to the presence of certain bioactive components categorised as polyphenols, present in its various parts. Present work describes a method for simultaneous detection and quantification of polyphenols present in the seed extract using HPLC and analyzing in-vitro anti-diabetic and anti-inflammatory potential of the extract. HPLC method was developed using Ultra HPLC with attached PDA detector, at a wavelength of 275 nm. The mobile phase used was acetonitrile: formic acid at gradient flow. Five polyphenols rutin, quercetin, apigenin, gallic acid, and catechin have been detected and quantified. The IC50 value (mg/mL; the concentration that causes a decrease in initial concentration by 50%) of ethyl acetate extract determined for α-Amylase, α-Glucosidase, and lipoxygenase enzymes was 471 mg/ml, 551 mg/ml and 524 mg/ml respectively.
Neeraj, N. Vasudeva *, S. Sharma, A. Duhan and Ravi
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India.
16 March, 2018
26 May, 2018
14 June, 2018
01 November, 2018