HPTLC FINGERPRINTING FOR SIMULTANEOUS QUANTIFICATION OF HARMINE, KAEMPFEROL, DIOSGENIN AND OLEIC ACID IN THE FRUIT EXTRACT OF TRIBULUS TERRESTRIS L. AND ITS FORMULATIONAbstract
A simple, accurate and reproducible HPTLC method has been developed for the simultaneous quantification of diosgenin, oleic acid, harmine and kaempferol from the methanolic extract of fruits of plant Tribulus terrestris L. and its marketed formulation. The CAMAG HPTLC systems with winCATS software used for the analysis. Double development with mobile phase Toluene: Ethyl acetate: Formic acid: Glacial acetic acid (2:1:1:0.75 v/v/v/v) on TLC plate silica gel 60 F 254. Detection and quantification of harmine at 366 nm, kaempferol at 270 nm, diosgenin at 201 nm and oleic acid at 580 nm after derivatization with anisaldehyde sulphuric acid reagent. The quantity of harmine was found to be 0.014% and 0.006%, kaempferol was 0.018% and 0.006%, oleic acid was 0.259% and 0.108% and diosgenin was 0.086% and 0.022% in plant and formulation respectively. The developed method was then validated in terms of specificity, linearity, LOD, LOQ, precision and recovery. The validated method was successfully applied for quantification of four components in a formulation containing Tribulus terrestris L. extract.
V. V. Vaidya, P. L. Kondalkar *, M. A. Shinde and S. Gotmare
Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai, Maharashtra, India.
31 October, 2017
31 March, 2018
12 June, 2018
01 July, 2018