HPTLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF OXYCLOZANIDE AND LEVAMISOLE HYDROCHLORIDE IN TABLET DOSAGE FORMAbstract
This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Oxyclozanide and Levamisole hydrochloride as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminium plates pre-coated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Toluene: acetone: ammonia (5:5:0.04 v/v/v). Densitometric evaluation of the separated zones was performed at 225 nm. The two drugs were satisfactorily resolved with R F values 0.28± 0.007 and 0.49 ± 0.009 for Oxyclozanide and Levamisole hydrochloride, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (400-1200 ng/band for Oxyclozanide) and (200-600 ng/band for Levamisole hydrochloride), precision (intra-day % RSD was 1.20 and inter-day % RSD was 1.51 for Oxyclozanide, and intra-day % RSD was 1.22 and inter-day % RSD was 1.52 for Levamisole hydrochloride), accuracy (0.556 for Oxyclozanide and 1.31 for Levamisole hydrochloride), and specificity in accordance with ICH guidelines.
H. Panchal *and M. Patel
Department of Pharmacognosy, Anand Pharmacy College, Anand, Gujarat, India.
04 February, 2017
07 April, 2017
25 June, 2017
01 September, 2017