HYDROCOTYLE SIBTHORPIODES EXTRACT LOADED IN CHITOSAN NANOCAPSULE AS AN EFFECTIVE ANTIBACTERIAL AGENT AGAINST FEW PREVALENT MICROBES IN WOUNDAbstract
Hydrocotyle sibthorpiodes is a known medicinal plant and is used for treatment against many bacterial and fungal pathogens in a conventional application form. The present study was intended to enhance the activity of H. sibthorpiodes plant extract against four bacterial species in combination with clove oil and silver nanoparticles by preparing a chitosan based nanocapsule that cause chronicity in wounds. The size of the synthesized nanocapsules was studied by Dynamic Light Scattering study (DLS) and SEM and was found to be 138.2 d.nm. However, the size of the loaded nanocapsule without AgNPs was obtained with a reduced size of 124.8 d.nm. The analysis of the result for the antibacterial study of the plant extract loaded nanocapsule with AgNPs was also found to be more than the extract alone and nanocapsules without AgNPs with an inhibition zone of 3.9 cm and 3.2 cm against both S. aureus as well as K. pneumonia respectively which was considered to be more effective than the conventional application of the plant extract for inhibiting the bacterial growth. The chitosan loaded plant extract may not only improve the efficacy of the plant active compounds but will also help in its targeted release thereby providing a good therapeutic benefit to the society with a low cost.