IN-VIVO STUDIES OF OPTIMIZED L-GLUTAMINE HYDROGEL FOR ORAL MUCOSITISAbstract
Mucositis is a foremost acute complication in the clinical setting, occurring in a large percentage of patients undergoing cytotoxic therapy. One of the major problems with mucositis is that the underlying mechanisms behind its development are not wholly understood, which makes it extremely difficult to develop effective interventions. Animal models provide a critical source of knowledge when sampling from patients is unavailable, or interventions are yet to be fully tested. The object of the study is to evaluate the potential effectiveness of optimized L-glutamine hydrogel formulation used in the buccal cavity; we used a rat model for oral mucositis induced by 5-fluorouracil. Studies have shown that the blend of mild abrasion of the cheek pouch and two doses of 5-fluorouracil (60- 40 mg/kg) induced oral mucositis and the reduction of body weight. Optimized hydrogel formulations CP4 and GSH14 shown significant weight gain as compared to the control group. This study shows that the prepared hydrogel is effective in oral mucositis and effective to control oral mucositis. The rationale selection and combination of the materials allow the design of suitable formulation for different purposes with simplified changes in compositions.