INVESTIGATION OF SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CEFOTAXIME SODIUM IN DIFFERENT BRANDS OF PHARMACEUTICAL PREPARATIONSAbstract
A simple, precise, and robust spectrophotometric method for the determination of Cefotaxime sodium has been investigated and validated. The proposed chemical reaction involves the reaction of primary amine of Cefotaxime sodium with nitrite solution in acidic medium to form a diazotized product, followed by the reaction of the diazotized product with suitable coupling reagent resulting in a colored product. Cefotaxime sodium has shown λmax 500nm. The method was found linear obeying Beer Lambert law at concentration range of 1-20 ppm. In the method detection limits was observed 0.744 ppm with quantification limit of 2.48 ppm and standard deviation of 0.248. In the present work an attempt was made to develop a simple, less expensive and more reliable method for the determination of Cefotaxime sodium in pure and pharmaceutical preparations.
S. Ali Ilyas, Md. Imran *, N. Kumar, J. Shah, M. Rasul Jan, S. Fazil and Md. Khalid
Department of Chemistry, University of Azad Jammu & Kashmir, Muzaffarabad, Pakistan
10 May, 2015
25 September, 2015
20 November, 2015
01 December, 2015