ISOLATION AND CHARACTERIZATION OF BIOFILM FORMING BACTERIA FROM URINARY TRACT INFECTED PATIENTSAbstract
A biofilm is a microbial determined sessile group portrayed by cells that are irreversibly appended to a substratum or interface or to each other. Biofilms are the main cause for increase in virulence and antibiotic resistance of microorganisms. Both gram positive and gram negative pathogens are known to produce biofilms. Due to the production of biofilm, the bacteria adhere to the surface strongly and are protected from any antibiotic agents. The present study is aimed at isolation and characterization of biofilm forming bacteria from UTI samples. Further, N-acylhomoserine lactone (AHL), an autoinducer responsible for the formation of biofilms in gram negative bacteria was characterized. The capacity of the UPEC isolates to form biofilms was analysed using biofilm assay. Further on, N-acylhomoserine lactone was extracted by liquid liquid extraction (LLE) and was estimated colorimetrically and spectroscopically by using fourier transform infrared spectroscopy (FT-IR). The UPEC isolates exhibited strong biofilm forming capacity. The AHL that was extracted showed a characteristic absorbance for C=O bond of lactone ring, and N=H and C-O bond of acyl chain.
P. K. Prasad, P. Sharma and P. Shlini *
Department of Chemistry (PG Biochemistry), Mount Carmel College Autonomous, Bangalore, Karnataka, India.
21 July, 2017
27 December, 2017
27 January, 2018
01 May, 2018