ISOLATION AND CHARACTERIZATION OF L-ASPARAGINASE EXTRACELLULAR ENZYME PRODUCING BACTERIA FROM INDUSTRIAL SOIL SAMPLESAbstract
Industrial soil contains a variety of L-Asparaginase producing microorganisms such as bacteria and fungi. Microorganisms can produce different types of enzymes which are frequently used in different types of industries for different purpose. In the present study, we focused on isolation of L-Asparaginase enzyme producing bacteria from industrial soil samples collected from different regions of Gwalior. These bacterial isolates were Salmonella sp., Proteus vulgaris, Bacillus subtilis which were identified based on colony morphology, Gram staining, biochemical tests and using selective and differential media. All the tested isolates were able to produce enzymes like L-Asparaginase. All the three bacteria which were isolated from soils of a different region of Gwalior able to produce L-Asparaginase enzyme. Maximum L-Asparaginase activity was shown in second (II) bacterial isolate Salmonella sp., which was 12.734 mg/ml. Salmonella sp. were not reported to show any type of allergenicity when their sequences were checked by bioinformatic tool Algpred. So, these bacterial isolates are considered as a good source of enzyme production and may be used at an industrial level. Thus from the present study, it may be concluded that microbes from soil sample can be a good source of industrially important enzymes.
R. S. Tomar, N. Sharma * and S. Kaushik
Amity Institute of Biotechnology, Amity University, Gwalior, Madhya Pradesh, India.
18 February 2019
20 May 2019
01 June 2019
01 November 2019