ISOLATION, PURIFICATION AND CHARACTERIZATION OF ANTIBACTERIAL PROTEINS FROM INDUCED LARVAE OF ANTHEREAE MYLITTA DRURY (DABA TV)Abstract
Antibacterial proteins are evolutionarily ancient, effect or molecules of innate immune defense and are ubiquitously found in all kingdoms. The present study deals with the induction of antibacterial proteins in the haemolymph of Anthereae mylitta drury (Daba TV) by injecting non-pathogenic Pseudomonas DAS-01. Purified protein fraction obtained on ammonium sulphate precipitation followed by ion exchange chromatography had shown antibacterial activity against Pseudomonas aeruginosa AC-3 and Pseudomonas DAS – 01. Flow through obtained in ion exchange chromatography found effective in inhibiting the growth of both P. aeruginosa AC-3 and Pseudomonas DAS – 01. In induced larvae, purified antibacterial peptides obtained by ion exchange chromatography on SDS-PAGE had shown a molecular weight of 16kDa and 24kDa which have antibacterial activity and were not observed in control.