ISOLATION, SCREENING AND CHARACTERIZATION OF L-ARGINASE PRODUCING SOIL BACTERIAAbstract
L-arginase (EC 18.104.22.168) is a therapeutic enzyme which catalyzes the hydrolysis of L-arginine to L-ornithine and urea. L-arginase is of keen interest due to its significance as an anti-cancer mediator against auxotrophic cancers. This enzyme is widely distributed and expressed in organisms like fungi, plants, non-chordates, and chordates, however very scarce report is available for L-arginase from bacteria. Therefore, the present study was aimed to isolate L-arginase producing bacteria from soil. Soil samples were collected from different locations in and around Karnatak University campus, Dharwad, Karnataka, India. L-arginase activity was confirmed by rapid plate assay screening using minimal arginine agar media and estimating the urea level colorimetrically. Among the sixty-eight isolates, twenty-nine of them were positive for the L-arginase production, but, the most potent isolate was selected, characterized, and identified in our study. The isolate was identified as Pseudomonas sp. strain PV1 (GenBank accession number MK459549.1).
P. D. Nadaf, A. G. Kulkarni and A. B. Vedamurthy *
PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
11 March 2019
22 June 2019
27 June 2019
01 July 2019