MURRAYA KOENIGII MEDIATED SILVER NANOPARTICLE SYNTHESIS AND ITS ACTIVITY AGAINST ENTERIC PATHOGENSAbstract
Objective: The objective of this research was a synthesis of silver nanoparticles from Murraya koenigii leaves in which the particles were characterized by various techniques and to evaluate the potential antibacterial activity of synthesized nanoparticles against various human pathogens. Methods: Murraya koenigii aqueous extract of leaves were prepared by the Soxhlet method, and the bioactive compounds were analyzed by phytochemical analysis. Silver nanoparticles were synthesized which are characterized by UV-visible spectroscopic analysis, XRD, TEM, FTIR analysis and antimicrobial activity was evaluated by agar well diffusion method. Results: Phytochemical analysis of Murraya koenigii aqueous extract of leaves showed the presence of alkaloids, phenols, flavonoids, terpenoids, coumarins, and steroids. The aqueous AgNo3 solution changed from green to dark brown color after incubation. UV-visible spectroscopic analysis showed the absorbance peak at around 420 nm which indicates the synthesis of Ag NPs. TEM image showed the formation of well-dispersed silver nanoparticles in the range of 5-25 nm. X-ray diffraction (XRD)-spectrum as evidenced by the peaks at 2θ values of such peaks 38.43°, 44.40°, and 67.57°, is corresponding to 111, 200 and 220 planes for silver, respectively. Silver nanoparticles showed the antimicrobial activity against Staphylococcus aureus (16mm), Escherichia coli (15mm), Salmonella sps. (15 mm), Bacillus sps. (16mm), Vibrio sps. (14mm), Enterococcus sps. (13mm) and Candida (21 mm). Conclusion: This study demonstrated that the silver nanoparticles from curry leaf extract showed that the remarkable antibacterial activity against human pathogens. The extract of Murraya koenigii has the bioactive compounds like alkaloids, phenols, flavonoids, terpenoids and it will become a drug for the therapy of infectious diseases.
R. Amutha and A. Sudha*
Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (Autonomous), Namakkal, Tamil Nadu, India.
22 July 2018
08 October 2018
08 January 2019
01 April 2019